Forty species of bats and around 59 species of mammals have been reported for the Park, among which are the gray-bellied night monkey (Aotus lemurinus), white-fronted capuchin monkey (Cebus albifrons), sloth (Bradypus variegatus), armadillo (Dasypus novemcinctus), red brocket deer (Mazama americana), jaguar (Panthera onca), ocelot (Leopardus pardalis), ant eater (Myrmecophaga tridactyla), as well as five species of marine mammals.
There are 396 species of birds including the little tinamou (Cripturellus soui), king vulture (Sarcoramphus papa), roadside hawk (Buteo magnirostris), yellow-headed caracara (Milvago chimachima) and pale-vented pigeon (Columba cayannensis).
Among the reptiles, there are four species of marine turtles (Caretta caretta, Chelonia mydas, Eretmochelys imbricata and Dermochelys coriacea) and boa constrictor. Recently, the presense of aguja caiman has been reported, making it one of the few populations in the Colombian Caribbean.
The diversity of marine species is also notable represented by mollusks, crustaceans, algae and coral reefs among others.